Phagocytic activity of peritoneal exudate cells from mice.

OELLERMANN, R. A., HOOGENHOUT, JOAN and CARTER, P. Phagocytic activity of peritoneal exudate cells from mice. Onderstepoort]. vet. Res. 39(4), 197-204 (1972). . Phago~ytic activity of peritoneal exudate cells in mice was stimulated by injection of 0,1 ml mineral 01l or varymg concentrations of lysolecithin. Optimal lysolecithin concentration was found to be 2 to 5 11-g pe~ mous~. The phagocytic activity of peritoneal exudate cells from mice was determined by spectral analysis of dioxane extracts of cells after incubation in the presence of polystyrene latex particles. Maximum uptake of latex particles occurred after an incubation period of 1 h. . Maximum phagocytic activity was observed in cells harvested 3 days after stimulation with mineral 01l and ap~rox1mately 4 days after stimulation with lysolecithin. Electron microscopy of these cells revealed a similar pattern of phagocytic activity. . Latex particles ~re inert and not metabolized by the cells and their uptake was therefore compared With the phagocytosis of bluetongue virus particles. Electron microscopic studies of the uptake of bluetongue vuus s~owed that although adsorption of the virus on the surface membrane occurred practically no ~hagocytos1s was observed 1 day after stimulation with lysolecithin. Maximum phagocytosis of virus particles occurred 3 to 4 days after stimulation with lysolecithin.